The scene is from the Nickelodeon animated series, The Penguins of Madagascar from the episode, Lemur See, Lemur Do
The Penguins take the robot lemur into their headquaters to analyze it and tells Mort to keep it secret from King Julien. After searching for the robot, King Julien asks Mort if he's seen it but he can tell Mort is lying. Mort says he's positive he hasn't seen it. King Julien then says he shouldn't then mind if he....TICKLES HIM. He then begins to tickle Mort while asking him to tell him where his robot is and he continues to tickle him until an explosion at Penguins HQ and Mort tells him where the robot is.